qmap file: >chr10 C 1 17 17 0 0 0 0 0 0 1.00 b bbbaabbbbbb_b`bbb From left to right: First raw: chr10:chromosome ID Second raw: C: Cytosine base 1: 1 read is mapped across this site, uniquely (defined as this alignment has the least mismatches comparing with other possible alignments, and in the whole genome only one such alignment exists) and is methylated (methylated here means, the read is from amplification products of a DNA molecule in vivo with this C methylated). 17: 17 reads are mapped across this site, uniquely and is unmethylated. 17: 17 reads are mapped across this site, uniquely and without methylation status (they are informative for another strand) 0: 0 read is mapped across this site, uniquely and is identified as a mismatch. 0: 0 read is mapped across this site, repeatedly (defined as this alignment has the least mismatches comparing with other possible alignments, but in the whole genome more than one such alignments exist) and is methylated (if the alignment is by chance correct). 0: 0 read is mapped across this site, repeatedly and is unmethylated. 0: 0 read is mapped across this site, repeatedly and not informative about methylation. 0: 0 read is mapped across this site, repeatedly and is identified as a mismatch 0: Sum of hits that reads repeatedly mapped across this site on the whole genome 1.00: Sum of (column 2-5 & 10)/Sum of (column 2-9). Estimation of the local complexity of this genomic region. b: Sequencing quality of the methylated reads uniquely mapped across this site(column 2) bbbaabbbbbb_b`bbb: Sequencing quality of the unmethylated reads uniquely mapped across this site(column 3)